Durum wheat improvement against fungal pathogens by using protein inhibitors of cell wall degrading enzymes
نویسندگان
چکیده
We report the use of three glycosidase inhibitors, the bean polygalacturonase inhibiting protein 2 (PvPGIP2), the kiwi pectin methyl esterase inhibitor (AcPMEI), and the Triticum aestivum xylanase inhibitor III (TAXI-III), to control leaf blotch and Fusarium Head Blight (FHB) symptoms caused by the fungal pathogens Bipolaris sorokiniana and Fusarium graminearum. We produced transgenic durum wheat lines by particle bombardment using these inhibitors singly or in combination. We pyramided these transgenes also by classical crossing of transgenic lines carrying a single transgene. Phyto-pathological tests performed in controlled conditions showed that the expression of these inhibitors has the potential to engineer a broadspectrum disease resistance in wheat.
منابع مشابه
اثر مهاری پروتئین‌های مهارکننده پلی گالاکتوروناز لوبیا بر آنزیم پلی گالاکتوروناز قارچ‌های بیماری‌زای Fusarium oxysporum و Ascochyta rabiei گیاه نخود
Plant pathogenic microorganisms produce a variety of enzymes capable of degrading different polysaccharides of the plant cell walls. Pathogens use these enzymes to penetrate and colonize host cells. Polygalacturonases are thought to be the first cell wall-degrading enzymes secreted by pathogens when they grow on plant cell walls. Oligogalacturonic acids with the polymerization degrees of 10 to ...
متن کاملاثر مهاری پروتئین‌های مهارکننده پلی گالاکتوروناز لوبیا بر آنزیم پلی گالاکتوروناز قارچ‌های بیماری‌زای Fusarium oxysporum و Ascochyta rabiei گیاه نخود
Plant pathogenic microorganisms produce a variety of enzymes capable of degrading different polysaccharides of the plant cell walls. Pathogens use these enzymes to penetrate and colonize host cells. Polygalacturonases are thought to be the first cell wall-degrading enzymes secreted by pathogens when they grow on plant cell walls. Oligogalacturonic acids with the polymerization degrees of 10 to ...
متن کاملThe expression of a bean PGIP in transgenic wheat confers increased resistance to the fungal pathogen Bipolaris sorokiniana.
A possible strategy to control plant pathogens is the improvement of natural plant defense mechanisms against the tools that pathogens commonly use to penetrate and colonize the host tissue. One of these mechanisms is represented by the host plant's ability to inhibit the pathogen's capacity to degrade plant cell wall polysaccharides. Polygalacturonase-inhibiting proteins (PGIP) are plant defen...
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Transgenic technology has demonstrated its usefulness in weed and insect control, but this technology has yet to expand much beyond these limited, special case scenarios. Along with stringent regulations, the primary limitation preventing expansion into new areas is finding efficacious genes. Fungal pathogens are particularly difficult to control in this regard as these organisms employ many of...
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